Beta-glucans Blocker mo le poloka o le Beta Glucan Pathway
Beta-glucans Blocker mo le poloka o le Beta Glucan Pathway
1. Fa'amatalaga o oloa
E lua auala i le limulus amoebocyte lysate lal reagent, o le auala C e faʻapitoa i le endotoxin ma le faʻailoga G e faʻapitoa i (1,3) - β-D-Glucans.Afai o le suʻega faʻataʻitaʻiga o loʻo i ai le β-1,3-Glucans, o le suʻega limulus (Endotoxin suʻega) o le ai ai faʻalavelave.β-G-Blocker poloka le gaioiga o le LAL i le β-1,3-Glucans, faʻatagaina le faʻateleina o le endotoxin i le suʻega LAL.Afai o faʻataʻitaʻiga suʻega o loʻo iai leβ-1,3-Glucan, pei ole cellulose, faʻaaogabeta-glucans polokao se manatu lelei le poloka o le β-1,3-Glucan faʻalavelave i le endotoxin ma le LAL reagent tali.
2. Fa'ailoga oloa
Endotoxin maualuga i lalo ole 0.005EU/ml
3. Fa'ailoga oloa ma fa'aoga
Suia le LAL Reagent Water e toe fa'afouina ai le LAL reagent, poloka le beta-glucan mediates factor G ala ma fa'amautinoa ole LAL reagent na'o le tali atu ile endotoxin.Mo faʻataʻitaʻiga faʻataʻitaʻiga o loʻo i ai le (1,3)- β-D-Glucan contamination.
Fa'amaumauga No. | Fa'amatalaga | Manatua | afifi |
BH10 | 50mM Tris pa'u, pH7.0, 10ml/vai | Fa'aoga e fa'alili ai fa'ata'ita'iga fa'atosina maualuga. | 10 fagu / pusa |
BH50 | 50mM Tris pa'u, pH7.0, 50ml/vai | Fa'aoga e fa'alili ai fa'ata'ita'iga fa'atosina maualuga. | 10 fagu / pusa |
BY10 | 10mM Magnesium Buffer, 10ml/vai | Faʻaaogaina e faʻatoʻilaloina ai le chelation effect. | 10 fagu / pusa |
BT10 | β-glucans poloka, 10ml/vai | Fa'aaoga e poloka ai le fa'alavelave β-glucan i fa'a'au a Amebocyte Lysate endotoxin. | 10 fagu / pusa |
PBS50 | PBS puipui endotoxin-sa'oloto, 50ml/vai | Fa'aoga e fufulu ai pusa fa'ata'ita'i, pe fa'afetaui le pH | 10 fagu / pusa |
PBS500 | PBS puipui endotoxin-sa'oloto, 500ml/vai | 1 fagu |
Tulaga Oloa
Ole ma'ale'ale ole Lyophilized Amebocyte Lysate ma le malosi ole Control Standard Endotoxin e fa'ata'ita'iina ile USP Reference Standard Endotoxin.O le Lyophilized Amebocyte Lysate reagent kits e sau ma fa'atonuga o oloa, Tusi Fa'amaonia o Su'esu'ega.