Pusa Chromogenic Endpoint EC64405

Bioendo EC Endotoxin Test Kits o lo'o tu'uina atu ai se ituaiga o fua mo su'ega su'ega endotoxin quantitative.O le endotoxin i totonu o le faʻataʻitaʻiga o loʻo faʻagaoioia ai se cascade o enzymes i le Amebocyte Lysate, o le enzyme faʻagaoioia e vaeluaina le mea faʻapipiʻi, tuʻuina atu se oloa lanu samasama ma le maualuga o le absorbance i le 405nm.Mo le End-point Chromogenic Endotoxin Test Kit e aunoa ma le Diazo Coupling, e manaʻomia se spectrophotometer masani poʻo se microplate faitau e faitau le miti ile 405nm.Ole spectrophotometer masani ma le galuega ole su'esu'ega kinetic, le sa'o o le pulea o le vevela ma le 405nm faamama, ona fa'aaoga lea ole spectrophotometer e mafai ona faitau le quantitative endotoxin i'uga o le EC kit.


Fa'amatalaga Oloa

Pusa Su'ega Chromogenic Endotoxin fa'ai'uga (e aunoa ma le Fa'atasia Diazo)

1. Fa'amatalaga Oloa

Pusa Su'ega Chromogenic Endotoxin pito i'uga(e aunoa ma le Diazo Coupling) e faia e ala i le faʻaopopoina o se vaifofo substrate peptide e leai ni lanu i totonu o le faʻafefiloi o Lyophilized Amebocyte Lysate ma faʻataʻitaʻiga faʻataʻitaʻi pe a maeʻa se vaitaimi faʻapitoa.Afai o le su'ega fa'ata'ita'iga e iai le endotoxin, o le a fa'atupula'ia le lanu samasama ile 96 lua microplate.O lona mitiia (λmax = 405nm) e fesoʻotaʻi ma le faʻaogaina o le endotoxin.E mafai ona fa'atatau ile fa'atatauga ole endotoxin ole fa'ata'ita'iga ile fua fa'atatau.

2. Fa'ailoga oloa

Fa'amatalaga Fa'amatalaga: 0.01-0.1 EU/ml, fa'afetaui taimi su'esu'e e tusa ma le 46 minute

0.1-1 EU / ml, faʻatusatusa taimi suʻega e tusa ma le 16 minute.

3. Oloa Fa'atusa ma Fa'aoga

End-point Chromogenic Endotoxin Test Kit (e aunoa ma le Diazo Coupling) ua fa'amoemoe mo le fa'aogaina i le In Vitro su'esu'ega ma le aofa'i o endotoxins siama leaga kalama.Ole vaifofo ole peptide substrate e leai ni lanu e fa'aopoopoina ile fa'afefiloi ole Lyophilized Amebocyte Lysate ma fa'ata'ita'iga fa'ata'ita'i pe a mae'a se vaitaimi fa'atupu.E tupu le lanu samasama pe a iai le fa'ata'ita'iga ole su'ega ole endotoxin.Ona fa'aaoga lea ole spectrophotometer masani po'o le plate reader e faitau ai le miti ile 405nm.End-point Chromogenic Endotoxin Test Kit (e aunoa ma le Diazo Coupling) e fetaui lelei mo ituaiga uma o faʻataʻitaʻiga faʻapitoa e pei ole polotini, tui, plasmid, DNA, RNA endotoxin assay.

Fa'aaliga:

Lyophilized Amebocyte Lysate (LAL) reagent na gaosia e Bioendo e faia mai le amebocyte lysate e maua mai i le toto o le paa solofanua.

Fa'amaumauga No.

Fa'amatalaga

Pusa i totonu

Ma'ale'ale

(EU/ml)

EC64405

 

Bioendo™ EC Endotoxin Su'ega Pusa (End-point Chromogenic Assay),

64 Su'ega/Pusa

 

2 Lyophilized Amebocyte Lysate, 1.7ml / vial;

2 Vai mo BET, 50ml / vial;

2 CSE;

4 Chromogenic Substrate, 1.7ml / vial;

0.1 – 1 EU/ml

EC64405S

0.01 – 0.1 EU/ml;

0.1 – 1 EU/m

Tulaga o oloa:

Ole ma'ale'ale ole Lyophilized Amebocyte Lysate ma le malosi ole Control Standard Endotoxin e fa'ata'ita'iina ile USP Reference Standard Endotoxin.O le Lyophilized Amebocyte Lysate reagent kits e sau ma fa'atonuga o oloa, Tusi Fa'amaonia o Su'esu'ega.

 

E mana'omia e le pusa su'esu'e endotoxin fa'ai'uga fa'apitoa le faitau microplate?

Bioendo EC64405 ma le EC64405S kit su'esu'ega ole tele ole endotoxin i'uga e ala ile faitau microplate masani.

Fa'ai'uga chromogenic endotoxin su'ega fa'asologa:

Endotoxin Free tube

Pyrogen Free Fautuaga

Pyrogen Free Microplates

Faitau masani Microplate

Endotoxin fa'ata'atia Fa'atonu Fa'afetaui (CSE10V)

Fa'aaogā le vai BET mo le toe fa'aleleia.

 



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