Pusa Chromogenic Endpoint EC80545
Bioendo EC Endotoxin Su'ega Pusa (End-point Chromogenic Assay, Diazo Coupling)
1. Fa'amatalaga Oloa
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) e maua ai se fua vave mo le aofaʻi o le endotoxin.O le endotoxin i totonu o le faʻataʻitaʻiga e faʻagaoioia ai se cascade o enzymes i Amebocyte Lysate, o le enzyme faʻagaoioia e vaeluaina le mea faʻapipiʻi, faʻasaʻo se mea lanu samasama.Ona mafai lea e le mea samasama ona tali atu i le diazo reagents e fausia ai mea lanu viole ma le maualuga o le miti i le 545nm.E mana'omia se spectrophotometer po'o se microplate mo le su'ega.O mea lanu violē e fa'atatau i le fa'aogaina o le endotoxin.Ona maua ai lea ole su'ega ole endotoxin ole su'esu'ega quantitative.
2. Fa'atauga Fa'atau
Ma'ale'ale Va'aiga: 0.01-0.1EU/ml (taimi su'esu'e e uiga ile 46 minute)
0.1-1.0EU/ml (taimi su'esu'e e tusa ma le 16 minute)
3. Oloa Fa'atusa ma Fa'aoga
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) o loʻo faʻamoemoe mo le faʻaaogaina i le In Vitro suʻesuʻega ma le aofaʻi o endotoxins bacterial gram-negative.Ole vaifofo ole peptide substrate e leai ni lanu e fa'aopoopo ile Lyophilized Amebocyte Lysate, ona fa'ata'ita'i lea o le paluga fa'ata'ita'iga.Afai o le fa'ata'ita'iga e iai le endotoxin, e sui le lanu o fa'afefiloi fa'ata'ita'iga.O le faʻafefe e fesoʻotaʻi ma le faʻaogaina o le endotoxin.O le mea lea e mafai ai ona fa'atatau le maualuga o le endotoxn i le fa'afefiloi o fa'ata'ita'iga i se pi'o masani.O le spectrophotometer masani ma le 540 - 545nm faamama ua lava lea e fuaina ai le endotoxin i la tatou EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).
Fa'aaliga:
Lyophilized Amebocyte Lysate (LAL) reagent na gaosia e Bioendo e faia mai le amebocyte lysate e maua mai i le toto o le paa solofanua.
| Fa'amaumauga No. | Fa'amatalaga | Pusa i totonu | Ma'ale'ale EU/ml |
| EC80545 | Bioendo™ EC Endotoxin Su'ega Pusa (Fa'ai'uga Chromogenic Su'ega, Diazo Coupling), 80 Su'ega/Pusa | 5 Lyophilized Amebocyte Lysate, 1.7ml / vial; 4Suavai mo BET, 50ml/vai; 5 CSE; 5 Chromogenic Substrate, 1.7ml / vial; 5 Diazo Reagent 1, 10ml / vial; 5 Diazo Reagent 2, 10ml / vial; 5 Diazo Reagent 3, 10ml / vial; | 0.1 – 1 EU/ml |
| EC80545S | 0.01 – 0.1 EU/ml; 0.1 – 1 EU/ml |
Ole ma'ale'ale ole Lyophilized Amebocyte Lysate ma le malosi ole Control Standard Endotoxin e fa'ata'ita'iina ile USP Reference Standard Endotoxin.O le Lyophilized Amebocyte Lysate reagent kits e sau ma fa'atonuga o oloa, Tusi Fa'amaonia o Su'esu'ega.
E mana'omia e le pusa su'esu'e endotoxin fa'ai'uga fa'apitoa le faitau microplate?
Bioendo EC80545 ma le EC80545S, e mafai ona faitau i le spectrophotometer masani.
Tu'u Au Feau
oloa fa'atatau
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Pusa Chromogenic Endpoint EC64405
Pusa Su'ega Chromogenic Endotoxin End-point (e aunoa ma le Diazo Coupling) 1. Fa'amatalaga o oloa Fa'ai'uga o le Pusa Su'ega Chromogenic Endotoxin (e aunoa ma le Diazo Coupling) e faia e ala i le fa'aopoopoina o se vaifofo peptide substrate e leai ni lanu i totonu o le fa'afefiloi o Lyophilized Amebocyte Lysate ma fa'ata'ita'iga fa'ata'ita'i pe a mae'a se fa'ata'ita'iga. taimi e a'afia ai.Afai o le su'ega fa'ata'ita'iga e iai le endotoxin, o le a fa'atupula'ia le lanu samasama ile 96 lua microplate.O lona mitiia (λmax = 405nm) e fesoʻotaʻi ma le faʻaogaina o le endotoxin.Endotoxin...
